4D3W
Crystal Structure of Epithelial Adhesin 1 A domain (Epa1A) from Candida glabrata in complex with the T-antigen (Galb1-3GalNAc)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-25 |
| Detector | MARRESEARCH MARMOSAIC 255 MM |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 55.010, 55.010, 139.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.610 - 1.500 |
| R-factor | 0.15918 |
| Rwork | 0.158 |
| R-free | 0.18295 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4asl |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.611 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.600 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.100 | 0.500 |
| Number of reflections | 35383 | |
| <I/σ(I)> | 13.4 | 4.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.9 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 291 | 5 MM CALCL2, 5 MM T-ANTIGEN (GAL-B-1, 3-GALNAC), 100 MM HEPES PH 7, 20% PEG 6000, 291 K |
