4D3Q

crystal structure of point mutated DUSP19 (R156A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-06
Detector	ADSC QUANTUM 315
Spacegroup name	P 31 2 1
Unit cell lengths	86.064, 86.064, 93.539
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 1.640
R-factor	0.1751
Rwork	0.174
R-free	0.19638
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3s4e
RMSD bond length	0.027
RMSD bond angle	2.440
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.670
High resolution limit [Å]	1.640	1.640
Rmerge	0.070	0.230
Number of reflections	49568
<I/σ(I)>	4.9	1.8
Completeness [%]	99.9	99.8
Redundancy	21.7	17.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.2M POTASSIUM SODIUM TARTRATE TETRAHYDRATE, 0.1M SODIUM CITRATE TRIBASIC DEHYDRATE (PH 5.6), 2.0M AMMONIUM SULFATE