4D3Q
crystal structure of point mutated DUSP19 (R156A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-06 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 86.064, 86.064, 93.539 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 1.640 |
| R-factor | 0.1751 |
| Rwork | 0.174 |
| R-free | 0.19638 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s4e |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.440 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.670 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.070 | 0.230 |
| Number of reflections | 49568 | |
| <I/σ(I)> | 4.9 | 1.8 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 21.7 | 17.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M POTASSIUM SODIUM TARTRATE TETRAHYDRATE, 0.1M SODIUM CITRATE TRIBASIC DEHYDRATE (PH 5.6), 2.0M AMMONIUM SULFATE |
