4D3G
Structure of PstA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 56.753, 56.753, 66.165 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.180 - 3.000 |
| R-factor | 0.24849 |
| Rwork | 0.246 |
| R-free | 0.29267 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m05 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.073 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.120 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.110 | 0.560 |
| Number of reflections | 2672 | |
| <I/σ(I)> | 27.2 | 7.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 38.2 | 38.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 200 MM NA MALONATE PH=4.6 AND 20% PEG3350 |
