4D2D
Structure of a tri peptide bound POT family peptide transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-02 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 103.380, 110.700, 110.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.556 - 2.522 |
| R-factor | 0.2005 |
| Rwork | 0.198 |
| R-free | 0.24220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aps |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.820 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.560 | 2.590 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.080 | 0.570 |
| Number of reflections | 21341 | |
| <I/σ(I)> | 10.4 | 2.2 |
| Completeness [%] | 98.7 | 99.3 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7 | 16-23 %(V/V) PEG 400, 0.1 M HEPES PH 7.0, 0.15-0.55 M NH4H2PO4 AND 10 MM TRI-ALANINE. CRYSTALS WERE GROWN BY THE LCP METHOD WITH 7.8 MAG AS HOSTING LIPID. |
