4D26
Crystal structure of the Bombyx mori Vasa helicase (E339Q) in complex with RNA,ADP and Pi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-18 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.960, 77.810, 107.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.780 - 2.100 |
| R-factor | 0.22872 |
| Rwork | 0.225 |
| R-free | 0.27707 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2db3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.196 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.130 | 0.670 |
| Number of reflections | 26616 | |
| <I/σ(I)> | 8.3 | 2.4 |
| Completeness [%] | 99.4 | 95.8 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M HEPES, PH 7.5, 25% PEG3350 |
