4D1T

High resolution structure of native tVIM-7 from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 43
Unit cell lengths	69.979, 69.979, 47.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.326 - 1.250
R-factor	0.1315
Rwork	0.131
R-free	0.14830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y8b
RMSD bond length	0.011
RMSD bond angle	1.386
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.320
High resolution limit [Å]	1.250	1.230
Rmerge	0.040	0.420
Number of reflections	61189
<I/σ(I)>	14.1	2
Completeness [%]	96.4	78.2
Redundancy	2.9	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1