4D1G
Crystal structure of the fiber head domain of the Atadenovirus snake adenovirus 1, native, second P212121 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.770, 96.750, 153.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.730 - 1.900 |
| R-factor | 0.17437 |
| Rwork | 0.173 |
| R-free | 0.23945 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d0v |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.385 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.390 |
| Number of reflections | 113650 | |
| <I/σ(I)> | 19.6 | 6.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 11.9 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 10 MM TRIS-HCL, 1.7 M AMMONIUM SULFATE, 0.085 M HEPES SODIUM SALT PH 7.5, 1.7%(V/V) PEG 400, 15%(V/V) GLYCEROL |
