4D1B

STRUCTURE OF MHP1, A NUCLEOBASE-CATION-SYMPORT-1 FAMILY TRANSPORTER, IN A CLOSED CONFORMATION WITH BENZYL-HYDANTOIN

Replaces:  2JLO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	287
Detector technology	CCD
Collection date	2010-10-11
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	95.614, 106.742, 107.852
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.706 - 3.800
R-factor	0.2857
Rwork	0.283
R-free	0.30810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4d1a
RMSD bond length	0.007
RMSD bond angle	1.094
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.870
High resolution limit [Å]	3.800	3.800
Rmerge	0.100	0.700
Number of reflections	10629
<I/σ(I)>	12.4	1.7
Completeness [%]	97.0	96
Redundancy	4.5	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		PROTEIN WAS CRYSTALLIZED FROM 27-33 % PEG 300, 0.1 M NACL AND 0.1M NA-PHOSPHATE (PH 7.0). BENZYL-HYDANTOIN WAS ADDED TO THE DROP AS A SATURATED SOLUTION.