4D10
Crystal structure of the COP9 signalosome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 31 |
| Unit cell lengths | 151.615, 151.615, 343.069 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.870 - 3.800 |
| R-factor | 0.19993 |
| Rwork | 0.199 |
| R-free | 0.22750 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.095 |
| Data reduction software | XDS |
| Data scaling software | XDS (AIMLESS) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.000 | 3.810 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.120 | |
| Number of reflections | 86875 | |
| <I/σ(I)> | 9.3 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.4 | CRYSTALS GROWN BY VAPOR DIFFUSION BY MIXING 9.3 MG/ML PROTEIN IN 50 MM HEPES PH 7.4, 200 MM NACL, 2 MM EQUALLY WITH 12% PEG 6000, 100 MM TRISODIUM CITRATE PH 5.4, 0.1 M LI2SO4, 10 MM UREA. |
