4D0Y
Crystal structure of DacB from Streptococcus pneumoniae D39
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.950, 37.634, 102.837 |
| Unit cell angles | 90.00, 97.98, 90.00 |
Refinement procedure
| Resolution | 101.840 - 2.000 |
| R-factor | 0.18409 |
| Rwork | 0.181 |
| R-free | 0.24263 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.888 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.920 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.180 | 0.800 |
| Number of reflections | 26483 | |
| <I/σ(I)> | 8.2 | 2.3 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 6.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.01 M ZINC CHLORIDE, 0.1 M TRIS-HCL PH 8.0, 14% (W/V) PEG 6000 |
