4D0U
Crystal structure of the fiber head domain of the Atadenovirus snake adenovirus 1, selenomethionine-derivative
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9768, 0.9791, 0.9793 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 149.540, 149.540, 149.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.330 - 1.600 |
| R-factor | 0.15884 |
| Rwork | 0.158 |
| R-free | 0.18854 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.542 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.090 | 0.400 |
| Number of reflections | 72960 | |
| <I/σ(I)> | 18.2 | 6.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.2 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 10 MM TRIS-HCL PH 8.5, 10 MM BETA-MERCAPTOETHANOL, 1.7 M AMMONIUM SULFATE, 0.085 M HEPES SODIUM SALT PH 7.5, 1.7%(V/V) POLYETHYLENE GLYCOL (PEG) 400, 15%(V/V) GLYCEROL |
