4D0Q
Hyaluronan Binding Module of the Streptococcal Pneumoniae Hyaluronate Lyase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-22 |
| Detector | MARRESEARCH MX300HE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.440, 60.800, 35.470 |
| Unit cell angles | 90.00, 95.53, 90.00 |
Refinement procedure
| Resolution | 35.310 - 1.200 |
| R-factor | 0.12878 |
| Rwork | 0.127 |
| R-free | 0.15939 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.630 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.300 | 1.260 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.040 | 0.430 |
| Number of reflections | 45456 | |
| <I/σ(I)> | 26.7 | 4.5 |
| Completeness [%] | 99.4 | 96.2 |
| Redundancy | 7.3 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 292 | BY THE SITTING DROP VAPOUR DIFFUSION METHOD BY MIXING EQUAL AMOUNTS OF THE PROTEIN SOLUTION WITH A MOTHER LIQUOR CONSISTING OF 18% (W/V) POLYETHYLENE GLYCOL 3,350 AND 0.1 M SODIUM ACETATE (PH 5.5) AT 292 K. |
