4D0P
Crystal structure of human CSN4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-25 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.660, 63.530, 75.800 |
| Unit cell angles | 90.00, 101.69, 90.00 |
Refinement procedure
| Resolution | 31.760 - 1.600 |
| R-factor | 0.1796 |
| Rwork | 0.179 |
| R-free | 0.19290 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | XDS (AIMLESS) |
| Phasing software | autoSHARP |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 1.610 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.050 | 1.350 |
| Number of reflections | 69613 | |
| <I/σ(I)> | 20.3 | 1.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.4 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 16 MG/ML PROTEIN IN 50 MM HEPES PH 7.4, 200 MM NACL, 2 MM DTT WAS MIXED EQUALLY WITH 100 MM HEPES PH 7.5, 4.3 M NACL. CRYSTALS WERE GROWN BY VAPOR DIFFUSION |
