4D0K

Complex of Chaetomium thermophilum PAN2 (WD40-CS1) with PAN3 (C-term)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-10
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	72.414, 93.568, 110.676
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.970 - 1.890
R-factor	0.1884
Rwork	0.187
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4czx
RMSD bond length	0.004
RMSD bond angle	0.866
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.630	2.000
High resolution limit [Å]	1.890	1.890
Number of reflections	60515
<I/σ(I)>	23.4	2.48
Completeness [%]	99.2	95.7
Redundancy	6.5	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		0.1M KCL, 0.1M HEPES PH7.5, 15% PEG6000