4D00
Haemagglutinin of H10N8 Influenza Virus Isolated from Humans in Complex with Human Receptor Analogue 6'SLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector | RIGAKU R-AXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.601, 183.169, 192.145 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 132.580 - 2.500 |
| R-factor | 0.20978 |
| Rwork | 0.209 |
| R-free | 0.23474 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.102 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.610 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.160 | 0.520 |
| Number of reflections | 390165 | |
| <I/σ(I)> | 6.21 | 2.06 |
| Completeness [%] | 92.8 | 90 |
| Redundancy | 3.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10% PEG 6000, 5% MPD AND 0.1 M HEPES PH 7.5 |
