4CZX

Complex of Neurospora crassa PAN2 (WD40) with PAN3 (C-TERM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-17
Detector	DECTRIS PILATUS 6M
Spacegroup name	C 2 2 21
Unit cell lengths	55.925, 160.651, 124.515
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.210 - 1.850
R-factor	0.169
Rwork	0.168
R-free	0.19670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bwx
RMSD bond length	0.007
RMSD bond angle	1.055
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (EP)
Refinement software	PHENIX (PHENIX.REFINE 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.210	1.960
High resolution limit [Å]	1.850	1.850
Number of reflections	48059
<I/σ(I)>	19.9	2.95
Completeness [%]	99.6	98.3
Redundancy	6.6	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		10% PEG 4000, 20% GLYCEROL, 0.1M MES/IMIDAZOLE (PH 6.5), 30MM SODIUM NITRATE, 30MM DI-SODIUM HYDROGEN PHOSPHATE, 30MM AMMONIUM SULFATE
1		6.5		10% PEG 4000, 20% GLYCEROL, 0.1M MES/IMIDAZOLE (PH 6.5), 30MM SODIUM NITRATE, 30MM DI-SODIUM HYDROGEN PHOSPHATE, 30MM AMMONIUM SULFATE