4CZV
Structure of the Neurospora crassa Pan2 WD40 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-17 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.990, 71.510, 77.120 |
| Unit cell angles | 90.00, 94.65, 90.00 |
Refinement procedure
| Resolution | 46.320 - 2.100 |
| R-factor | 0.1835 |
| Rwork | 0.182 |
| R-free | 0.21620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4CZX CHAIN A |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.827 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.700 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 38536 | |
| <I/σ(I)> | 12.2 | 2.18 |
| Completeness [%] | 99.5 | 97.1 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M AMMONIUM CHLORIDE, 18% PEG3350, 2% TERT-BUTANOL |
