4CZN
Crystal structure of the extralong fungal manganese peroxidase from Ceriporiopsis subvermispora
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-06 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 108.560, 108.560, 68.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.626 - 1.199 |
| R-factor | 0.1353 |
| Rwork | 0.134 |
| R-free | 0.15110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m5q |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.244 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.270 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.080 | 1.200 |
| Number of reflections | 128076 | |
| <I/σ(I)> | 18.7 | 0.02 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 8.8 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 18% PEG 4000, 100MM NA-ACETATE PH 4.6 |
