4CZD
Sirohaem decarboxylase AhbA/B - an enzyme with structural homology to the Lrp/AsnC transcription factor family that is part of the alternative haem biosynthesis pathway.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-09 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.850, 78.620, 151.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.770 - 2.230 |
| R-factor | 0.1994 |
| Rwork | 0.198 |
| R-free | 0.23550 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SOLVE-RESOLVE |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.800 | 2.290 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.050 | 0.690 |
| Number of reflections | 32358 | |
| <I/σ(I)> | 13.1 | 2 |
| Completeness [%] | 97.6 | 89.6 |
| Redundancy | 5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 26% PEG4K, 0.2M AMMONIUM ACETATE, 0.1M NA ACETATE PH 5.0, 0.01M BACL | ||
| 1 | 5 | 26% PEG4K, 0.2M AMMONIUM ACETATE, 0.1M NA ACETATE PH 5.0, 0.01M BACL |
