4CZB
Structure of the sodium proton antiporter MjNhaP1 from Methanocaldococcus jannaschii at pH 8.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Collection date | 2012-07-13 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.437, 102.544, 132.096 |
| Unit cell angles | 90.00, 105.63, 90.00 |
Refinement procedure
| Resolution | 32.000 - 3.500 |
| Rwork | 0.252 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cz8 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 3.720 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.090 | 0.570 |
| Number of reflections | 58249 | |
| <I/σ(I)> | 6 | 1.7 |
| Completeness [%] | 99.3 | 99.2 |
| Redundancy | 7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | pH 8.2 |
