4CZA
Structure of the sodium proton antiporter PaNhaP from Pyrococcus abyssii with bound thallium ion.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-11 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.056, 107.402, 99.796 |
| Unit cell angles | 90.00, 96.37, 90.00 |
Refinement procedure
| Resolution | 49.599 - 3.200 |
| R-factor | 0.2507 |
| Rwork | 0.248 |
| R-free | 0.29750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cz8 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.714 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.600 | 3.400 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.040 | 0.620 |
| Number of reflections | 34763 | |
| <I/σ(I)> | 13.4 | 1.8 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 17.1 | 17.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8.0 |
