4CZ8
Structure of the sodium proton antiporter PaNhaP from Pyrococcus abyssii at pH 8.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-11 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.493, 107.944, 107.928 |
| Unit cell angles | 90.00, 95.19, 90.00 |
Refinement procedure
| Resolution | 48.487 - 3.150 |
| R-factor | 0.2407 |
| Rwork | 0.239 |
| R-free | 0.27560 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.758 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXD |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.350 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.030 | 0.500 |
| Number of reflections | 38952 | |
| <I/σ(I)> | 11.9 | 1.5 |
| Completeness [%] | 99.5 | 99.2 |
| Redundancy | 10.8 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8.0 |
