4CZ7

Truncated tetramerization domain of zebrafish p53 (crystal form III)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Spacegroup name	C 1 2 1
Unit cell lengths	71.734, 45.717, 55.486
Unit cell angles	90.00, 92.60, 90.00

Refinement procedure

Resolution	27.700 - 1.100
R-factor	0.14415
Rwork	0.143
R-free	0.16588
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.240
Refinement software	REFMAC (5.8.0069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.700	1.160
High resolution limit [Å]	1.100	1.100
Rmerge	0.060	0.170
Number of reflections	68831
<I/σ(I)>	12.1	6.5
Completeness [%]	94.8	87.4
Redundancy	3.5	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	SITTING DROP VAPOR DIFFUSION AT 20 DEGREE C; PROTEIN SOLUTION: 17 MG/ML IN 20 MM TRIS PH 7.5, 50 MM NACL, 5 MM DTT; CRYSTALLIZATION BUFFER: 1.8 M SODIUM/POTASSIUM PHOSPHATE, PH 5.0.