4CYV
Structure of the A_mallard_Sweden_51_2002 H10 Avian Haemmaglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.612, 227.989, 68.716 |
| Unit cell angles | 90.00, 110.32, 90.00 |
Refinement procedure
| Resolution | 113.990 - 2.300 |
| R-factor | 0.21575 |
| Rwork | 0.214 |
| R-free | 0.25204 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ti8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.606 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.150 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.060 | 0.520 |
| Number of reflections | 84779 | |
| <I/σ(I)> | 11.9 | 2.3 |
| Completeness [%] | 98.4 | 99 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 18% PEG 2000MME AND 0.1 M BICINE PH 9.0 |
