4CYE
Crystal structure of avian FAK FERM domain FAK31-405 at 3.2A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-16 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.934, 146.128, 69.052 |
| Unit cell angles | 90.00, 96.98, 90.00 |
Refinement procedure
| Resolution | 73.060 - 3.200 |
| R-factor | 0.17633 |
| Rwork | 0.173 |
| R-free | 0.23977 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aeh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.133 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.000 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.080 | 0.330 |
| Number of reflections | 13590 | |
| <I/σ(I)> | 7.9 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 18%PEG4000, 325MM MGCL2, 0.1M TRIS PH8.5, 10MM TCEP |
