4CY8
2-hydroxybiphenyl 3-monooxygenase (HbpA) in complex with FAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2013-12-12 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 1 |
| Unit cell lengths | 80.000, 96.180, 102.160 |
| Unit cell angles | 114.09, 95.80, 109.31 |
Refinement procedure
| Resolution | 59.530 - 2.030 |
| R-factor | 0.17109 |
| Rwork | 0.169 |
| R-free | 0.20246 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ihg |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.749 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.530 | 2.080 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.070 | 0.430 |
| Number of reflections | 146673 | |
| <I/σ(I)> | 5.4 | 2.1 |
| Completeness [%] | 90.0 | 92 |
| Redundancy | 2.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 18% (W/V) PEG 3350, 0.15 M KSCN, 1 MM FAD IN BIS-TRIS PROPANE BUFFER AT PH 5.5, WITH A PROTEIN CONCENTRATION OF 10 MG ML-1 |
