4CY5
Crystal structure of the NSL1-WDS-NSL2 complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.480, 86.210, 47.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.110 - 2.300 |
| R-factor | 0.20881 |
| Rwork | 0.207 |
| R-free | 0.24471 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g99 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.145 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.730 |
| Number of reflections | 15438 | |
| <I/σ(I)> | 15.3 | 2.3 |
| Completeness [%] | 99.4 | 98.9 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.6 | 20% (W/V) PEG 3350, 0.2 M DI-AMMONIUM TARTRATE, PH 6.6 |
