4CY3
Crystal structure of the NSL1-WDS complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-30 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 78.740, 98.850, 80.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.810 - 1.400 |
| R-factor | 0.17995 |
| Rwork | 0.179 |
| R-free | 0.18988 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g99 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.015 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.460 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.110 | 0.870 |
| Number of reflections | 60987 | |
| <I/σ(I)> | 13.9 | 2.4 |
| Completeness [%] | 98.9 | 91.2 |
| Redundancy | 7.3 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 20% (W/V) PEG 3350, 0.2 M POTASSIUM SODIUM TARTRATE, PH 7.2. |
