4CW1
COMPLEX OF A B14 CHICKEN MHC CLASS I MOLECULE AND A 9MER CHICKEN PEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Collection date | 2011-11-11 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.200, 90.580, 144.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.380 - 2.580 |
| R-factor | 0.286 |
| Rwork | 0.286 |
| R-free | 0.29090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2YF6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.840 |
| Data reduction software | XDS (THROUGH XIA2) |
| Data scaling software | Aimless (THROUGH XIA2) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.380 | 2.650 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.140 | 0.590 |
| Number of reflections | 26279 | |
| <I/σ(I)> | 8.8 | 2.3 |
| Completeness [%] | 99.3 | 98.4 |
| Redundancy | 5.5 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.1M MIB BUFFER, PH 5.0, 25% W/V PEG 1500 |
