4CW0
Crystal structure of cofactor-free urate oxidase anaerobically complexed with 9-methyl uric acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-22 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 79.770, 94.990, 104.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.250 - 1.500 |
| R-factor | 0.11817 |
| Rwork | 0.116 |
| R-free | 0.15738 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.464 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.540 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.100 | 0.560 |
| Number of reflections | 62422 | |
| <I/σ(I)> | 7.3 | 2.3 |
| Completeness [%] | 98.5 | 99.4 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 20 MG/ML UOX IN 50 MM TRIS-HCL PH 8.0 SATURATED WITH 9-METHYL URIC ACID 8% PEG 8000, 50 MM TRIS-HCL PH 8.0 CRYSTALS GROWN ANAEROBICALLY |
