4CVO
Crystal structure of the N-terminal colied-coil domain of human DNA excision repair protein ERCC-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 37.444, 37.444, 321.268 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.696 - 1.850 |
| R-factor | 0.2373 |
| Rwork | 0.236 |
| R-free | 0.25530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.925 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.900 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.060 | 1.150 |
| Number of reflections | 12739 | |
| <I/σ(I)> | 18.3 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.8 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.6 M MAGNESIUM SULFATE, 0.1 M MES PH 6.6 |
