4CVN
Structure of the Fap7-Rps14 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.050, 139.550, 83.080 |
| Unit cell angles | 90.00, 105.51, 90.00 |
Refinement procedure
| Resolution | 44.766 - 2.121 |
| R-factor | 0.1741 |
| Rwork | 0.172 |
| R-free | 0.21250 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.285 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 2.250 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.070 | 0.660 |
| Number of reflections | 312402 | |
| Completeness [%] | 99.4 | 97.3 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M MGCL2, 0.1 M TRIS-HCL PH 8.5, 25 %(W/V) POLYETHYLENE GLYCOL 3350 |
