4CVC
Crystal structure of quinone-dependent alcohol dehydrogenase from Pseudogluconobacter saccharoketogenenes with zinc in the active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-10-30 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.220, 87.160, 57.000 |
| Unit cell angles | 90.00, 90.29, 90.00 |
Refinement procedure
| Resolution | 72.080 - 1.830 |
| R-factor | 0.16222 |
| Rwork | 0.161 |
| R-free | 0.18405 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cvb |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.146 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 1.930 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.080 | 0.240 |
| Number of reflections | 54822 | |
| <I/σ(I)> | 14.6 | 6.2 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 18-21% PEG550, 20 MM ZN(AC)2, 100 MM PCB BUFFER PH 6-7, HANGING DROP |
