4CV5
yeast NOT1 CN9BD-CAF40 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-19 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 188.339, 188.339, 126.689 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.292 - 3.807 |
| R-factor | 0.2773 |
| Rwork | 0.276 |
| R-free | 0.29520 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.885 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.290 | 4.010 |
| High resolution limit [Å] | 3.810 | 3.810 |
| Rmerge | 0.010 | 1.500 |
| Number of reflections | 22861 | |
| <I/σ(I)> | 18 | 2.7 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 25.5 | 23.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 50 MM HEPES PH 7.0, 3 M NACL |
