4CUT
Crystal structure of human BAZ2B in complex with fragment-5 N09428
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-10 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 83.495, 97.084, 58.127 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.736 - 1.835 |
R-factor | 0.17 |
Rwork | 0.169 |
R-free | 0.19660 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.064 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.060 | 1.880 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.080 | 0.860 |
Number of reflections | 20777 | |
<I/σ(I)> | 15.7 | 2 |
Completeness [%] | 98.7 | 89.3 |
Redundancy | 6.6 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 30% PEG600, 0.1M MES PH 6.0 |