4CUO
Banyan peroxidase with glycosylation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0, 1.44163 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 73.115, 73.115, 164.596 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 63.320 - 1.670 |
| R-factor | 0.15776 |
| Rwork | 0.157 |
| R-free | 0.18029 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.145 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.760 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.050 | 0.990 |
| Number of reflections | 60369 | |
| <I/σ(I)> | 22.9 | 2.4 |
| Completeness [%] | 99.5 | 97.7 |
| Redundancy | 7.4 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 1 UL PROTEIN (30 MG/ML IN 20 MM TRIS/HCL, PH 8.0) PLUS 1 UL RESERVOIR (1.3 M AMMONIUM SULFATE, 1.0 M LITHIUMSULFATE), CRYOSOLUTION: RESERVOIR INCLUDING 20% GLYCEROL, XE-DERIVATIZATION: 1 MIN 4 MPA XE FOR CRYSTAL IN CRYOSOLUTION. |
