4CUJ
Structure of Salmonella D-Lactate Dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-10-22 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.680, 137.210, 145.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.790 - 1.880 |
| R-factor | 0.19323 |
| Rwork | 0.191 |
| R-free | 0.23347 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dxy |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.828 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.460 | 1.980 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.110 | 0.570 |
| Number of reflections | 123429 | |
| <I/σ(I)> | 13.4 | 3.8 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M [ IMIDAZOLE; SODIUM CACODYLATE; MES(ACID); BIS-TRIS ] PH 6.5; 0.09M NANO3; NA2HPO4; (NH4)2SO4; 30% ETHYLENE GLYCOL; PEG 8K |
