4CUA
Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 70.290, 70.290, 159.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.540 |
| R-factor | 0.16224 |
| Rwork | 0.161 |
| R-free | 0.18893 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.873 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX (CDE) |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.330 | 1.630 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.080 | 0.400 |
| Number of reflections | 59949 | |
| <I/σ(I)> | 21.9 | 7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 16.6 | 16.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
