4CU7

Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 43 2 2
Unit cell lengths	116.780, 116.780, 219.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	116.780 - 2.200
R-factor	0.1524
Rwork	0.151
R-free	0.18173
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.385
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.930	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.090	0.210
Number of reflections	77385
<I/σ(I)>	14.2	6.7
Completeness [%]	99.6	93.7
Redundancy	7.3	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1