4CU6
Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 116.770, 116.770, 220.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 116.770 - 2.700 |
| R-factor | 0.18031 |
| Rwork | 0.178 |
| R-free | 0.22126 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.416 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.940 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.110 | 0.420 |
| Number of reflections | 42431 | |
| <I/σ(I)> | 10.9 | 3.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
