4CU2

C-terminal domain of CTP1L endolysin mutant V195P that reduces autoproteolysis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	I 2 2 2
Unit cell lengths	44.932, 48.821, 77.226
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.540 - 2.110
R-factor	0.17617
Rwork	0.172
R-free	0.26382
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	CD27L C-TERMINAL DOMAIN
RMSD bond length	0.006
RMSD bond angle	1.034
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.100	2.100
Rmerge	0.020
Number of reflections	4489
<I/σ(I)>	48.9
Completeness [%]	92.0
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		20MM TRIS PH8.0 AND 6 % PEG 8000