4CTI
Escherichia coli EnvZ histidine kinase catalytic part fused to Archaeoglobus fulgidus Af1503 HAMP domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-10-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.060, 76.620, 97.370 |
| Unit cell angles | 90.00, 107.03, 90.00 |
Refinement procedure
| Resolution | 38.830 - 2.847 |
| R-factor | 0.2587 |
| Rwork | 0.258 |
| R-free | 0.27650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zrv |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.710 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.800 | 3.020 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.090 | 0.590 |
| Number of reflections | 23475 | |
| <I/σ(I)> | 8.87 | 2.09 |
| Completeness [%] | 99.2 | 97.1 |
| Redundancy | 3.32 | 3.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M LITHIUM ACETATE AND 20 %(W/V) PEG 3350 |
