4CTD
X-ray structure of an engineered OmpG loop6-deletion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-01 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.940, 60.060, 59.760 |
| Unit cell angles | 70.45, 95.24, 70.58 |
Refinement procedure
| Resolution | 44.210 - 3.200 |
| R-factor | 0.2409 |
| Rwork | 0.238 |
| R-free | 0.28890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wvp |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.311 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.210 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.150 | 0.540 |
| Number of reflections | 11053 | |
| <I/σ(I)> | 5.3 | 2 |
| Completeness [%] | 92.8 | 93.9 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS/HCL, PH 8.5, 20% (V/V) ETOH; 8 MG/ML OMPG IN 20 MM TRIS/HCL, PH 8.0, 250 MM NACL, 10% (V/V) GLYCEROL, 0.4% (V/V) C8E4 |
