4CT9
Competence or damage-inducible protein CinA from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.190, 93.790, 132.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.020 - 2.140 |
R-factor | 0.19894 |
Rwork | 0.197 |
R-free | 0.24152 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a9s |
RMSD bond length | 0.011 |
RMSD bond angle | 1.521 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.000 | 2.200 |
High resolution limit [Å] | 2.140 | 2.140 |
Rmerge | 0.090 | 0.580 |
Number of reflections | 50324 | |
<I/σ(I)> | 9.6 | 2.1 |
Completeness [%] | 98.5 | 97.2 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.2 M SODIUM SULFATE 0.1 M BIS-TRIS PROPANE 20 % (W/V) PEG 3350 |