4CT8
Competence or damage-inducible protein CinA from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.970, 93.550, 132.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.460 - 2.160 |
| R-factor | 0.19072 |
| Rwork | 0.188 |
| R-free | 0.24073 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a9s |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.518 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.000 | 2.220 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.090 | 0.600 |
| Number of reflections | 49274 | |
| <I/σ(I)> | 12.1 | 2.2 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 4.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M SODIUM SULFATE 0.1 M BIS-TRIS PROPANE 20 % (W/V) PEG 3350 |
