4CT4
CNOT1 MIF4G domain - DDX6 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.531, 120.014, 78.469 |
| Unit cell angles | 90.00, 104.01, 90.00 |
Refinement procedure
| Resolution | 64.290 - 2.300 |
| R-factor | 0.1687 |
| Rwork | 0.166 |
| R-free | 0.21980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4GML AND PDB ENTRY 2WAX |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.185 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.150 | 2.410 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.070 | 0.660 |
| Number of reflections | 66414 | |
| <I/σ(I)> | 11.6 | 2.2 |
| Completeness [%] | 98.9 | 93 |
| Redundancy | 4.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 100 MM MES PH 6.5, 300 MM MGCL2 |
