4CSW
Rhodothermus marinus YCFD-like ribosomal protein L16 Arginyl hydroxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.300, 87.690, 90.610 |
| Unit cell angles | 90.00, 113.10, 90.00 |
Refinement procedure
| Resolution | 30.206 - 2.821 |
| R-factor | 0.1712 |
| Rwork | 0.168 |
| R-free | 0.20960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ccl |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.818 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.210 | 2.890 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Rmerge | 0.120 | 0.800 |
| Number of reflections | 27522 | |
| <I/σ(I)> | 7.6 | 2.1 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.66MM PROTEIN, 1MM MNCL2, 2MM IOX3, 50%(V/V) TACSIMATE, pH 7.5 |
