4CSR

High resolution crystal structure of the histone fold dimer (NF-YB)-(NF-YC)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210
Spacegroup name	P 21 21 21
Unit cell lengths	51.670, 60.800, 61.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.180 - 1.500
R-factor	0.1466
Rwork	0.145
R-free	0.18432
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n1j
RMSD bond length	0.007
RMSD bond angle	1.116
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.180	1.580
High resolution limit [Å]	1.500	1.500
Rmerge	0.060	0.360
Number of reflections	31692
<I/σ(I)>	14.7	4
Completeness [%]	99.7	100
Redundancy	4.5	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.2		28% PEG 4K, 100 MM TRIS PH 8.2, 200 MM MG(OAC)2, 100 MM NA(OAC)