4CSK
human Aquaporin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2013-02-24 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 89.280, 89.280, 174.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.490 - 3.280 |
| R-factor | 0.2368 |
| Rwork | 0.234 |
| R-free | 0.29780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j4n |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.360 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.500 | 3.670 |
| High resolution limit [Å] | 3.280 | 3.280 |
| Rmerge | 0.120 | 0.680 |
| Number of reflections | 5560 | |
| <I/σ(I)> | 22.1 | 3.9 |
| Completeness [%] | 97.3 | 96.9 |
| Redundancy | 25.3 | 16.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | pH 9 |
