4CSK
human Aquaporin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2013-02-24 |
Detector | DECTRIS PILATUS |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 89.280, 89.280, 174.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.490 - 3.280 |
R-factor | 0.2368 |
Rwork | 0.234 |
R-free | 0.29780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j4n |
RMSD bond length | 0.010 |
RMSD bond angle | 1.360 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 12.500 | 3.670 |
High resolution limit [Å] | 3.280 | 3.280 |
Rmerge | 0.120 | 0.680 |
Number of reflections | 5560 | |
<I/σ(I)> | 22.1 | 3.9 |
Completeness [%] | 97.3 | 96.9 |
Redundancy | 25.3 | 16.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9 | pH 9 |