4CSI
Crystal structure of the thermostable Cellobiohydrolase Cel7A from the fungus Humicola grisea var. thermoidea.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-01 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.931, 85.266, 135.854 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.320 - 1.800 |
| R-factor | 0.16659 |
| Rwork | 0.164 |
| R-free | 0.20975 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cel |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.361 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.700 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.090 | 0.410 |
| Number of reflections | 65221 | |
| <I/σ(I)> | 7.1 | 2.68 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | PROTEIN WAS CRYSTALLIZED FROM 22 % (W/V) PEG 8000, 0.2 M AMMONIUM SULPHATE, 20 MM TRIS HCL, PH 7.0 |
